Notes 20100428.092233 Conference Rainbow Trout
From SnOwy - Ed's Wiki Notebook
Contents |
Rad9-Rad1-Hus1 (9-1-1 complex) genome checkpoints
- rainbow trout toxicology
- in vitro rainbow trout cell lines
- G1/S Checkpoint -- replication mechanism checks, verifies DNA integrity
- checkpoint proteins used: single strand breakages, double stranded breaks, mitosis etc.,
CHK2 protein
- rainbow trout CHK2 (Steinmoeller et al., 2009)
- polyclonal anti-rtCHK2 antibodies
- in vivo / in vitro: upgregulated tissue damage
9-1-1 Complex
- a trimer -- creates a double-alpha-helix hexagonal ring
- Replication factor C (RFC) RAD17 -- Single stranded DNA response
- recombinant RAD1 protein overexpressed (synthetic biology, with microbes)
- rabbit innoculated with this protein (creation of antibodies)
Questions
- is RAD1 highly conserved in eukarya?
- do we expect any deleterious effects on the bunny?
- not sure, hasn't been checked yet
- in human, it's roughly 51% identical
