Notes 20100428.095446 Heat Shock Protein 70 Rainbow Trout Blood Cellular Stress Marker

From SnOwy - Ed's Wiki Notebook

Contents 1 Cellular Stress Marker, Heat Shock Protein 70, Rainbow Trout 2 Extracelular HSP70 2.1 Leader Sequence 3 Heat Shock Exposures 3.1 Acute Heat Shock 3.2 Cadmium 4 Results 4.1 Heat 4.2 Cadmium, MWWE 5 Conclusion

Cellular Stress Marker, Heat Shock Protein 70, Rainbow Trout

• Speaker: Lynsi Henrickson
• heat shock protein: highly conserved, found throughout cellular environment
• stress increases activates heat shock proteins, cytoprotective
• what is meant by 'activates'? transcription, translation or allosteric change?

Extracelular HSP70

• discovered recently
• observed from glial cells-- taken up by neuron neighbours
• thought to be a danger signaller
• has antigenic properties
• no leader sequence (?)
• not sure how this protein is emitted into extracellular environment
• lack of information on extracellular hsp
• and lack of information on regulation in non-mammalian vertebrates

Leader Sequence

Heat Shock Exposures

• acute stress-- heat shock study
• chronic stress-- sublethal cadmium
• chronic MWWE (municipal waste water exposure)

Acute Heat Shock

• added 15ºC for an hour

Cadmium

• added

Results

Heat

• discovered that presence of HSP70 is increased after 24 hours
  1. brain
  2. liver
  3. RBC

Cadmium, MWWE

• HSC70: no significant increase
• Cd: transient increase for HSP70
• MWWE: long term increase for HSP70

Conclusion

• heat shock protein HSP70 in blood stream is good marker for heat stress