Notes 20101012 Moffatt BIOL 208 TA Meeting

From SnOwy - Ed's Wiki Notebook

Arrived two minutes late, having just come from class.

Tutorial

Keep

• NEB cutter -- do a demo while they can follow along
• important key issues -- vectors: lambda, cosmids (sizes of the insert -- lambda phage, multiple end-to-end inserts for cosmids)

Defer

• advantages and disadvantages of certain vectors / plasmids -- cos sites etc.?
• lambda
• what in-frame means
  • useful for later so that they can understand what it means for a reporter gene to be in-frame
  • vectors that have epitope tags
  • examples -- will a given insertion providing a given restriction enzyme be in-frame?
• week for questions used?
• transcriptional and translational fusions
• electrocompetent cells
• plaques
• vectors, other kinds of vectors