Notes 20101012 Moffatt BIOL 208 TA Meeting
From SnOwy - Ed's Wiki Notebook
Arrived two minutes late, having just come from class.
Tutorial
Keep
- NEB cutter -- do a demo while they can follow along
- important key issues -- vectors: lambda, cosmids (sizes of the insert -- lambda phage, multiple end-to-end inserts for cosmids)
Defer
- advantages and disadvantages of certain vectors / plasmids -- cos sites etc.?
- lambda
- what in-frame means
- useful for later so that they can understand what it means for a reporter gene to be in-frame
- vectors that have epitope tags
- examples -- will a given insertion providing a given restriction enzyme be in-frame?
- week for questions used?
- transcriptional and translational fusions
- electrocompetent cells
- plaques
- vectors, other kinds of vectors